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Colorimetric liposome lysis for assay of anti-streptolysin O antibody by K Kubotsu; I Ushio; K Yoshikawa; M Kida; K Ishikawa; S Matsuura; I Sakurabayashi is a Medicine article available to read on EtoBox.

What is Colorimetric liposome lysis for assay of anti-streptolysin O antibody about?

## Abstract We developed an automated colorimetric assay based on liposome lysis for measurement of the titer of anti-streptolysin O antibodies (ASO) in human sera by using liposomes in which alkaline phosphatase (EC 3.1.3.1) was entrapped. The assay involved the inhibition by ASO of liposome lysis caused by streptolysin O. The procedure was fully automated for use with a routine clinical automated analyzer. The inter- and intra-assay precision showed that results were reproducible. Potential interfering substances such as bilirubin, hemoglobin, and triglycerides did not affect the results. With this assay, results correlated well with those by the Rantz-Randall method and the latex agglutination method (r = 0.900 and 0.890, respectively). This automated colorimetric assay for ASO should be of use for diagnosing streptococcal infections.

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Author

K Kubotsu; I Ushio; K Yoshikawa; M Kida; K Ishikawa; S Matsuura; I Sakurabayashi

Publisher

American Association for Clinical Chemistry; American Association for Clinical Chemistry, Inc.; Oxford University Press (OUP); American Association for Clinical Chemistry (AACC); Test accounts (ISSN 0009-9147)

Published

1990

Language

EN

Field

Medicine (Health Sciences)