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Effect of buffer composition and preparation protocol on the dispersion stability and interfacial behavior of aqueous DPPC dispersions by Sook Heun Kim; Yoonjee Park; Sadis Matalon; Elias I. Franses is a Materials Science article available to read on EtoBox.

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The effect of the buffer composition and the preparation protocol on the dynamic surface tension (DST) and vesicle sizes of aqueous dipalmitoylphosphatidylcholine (DPPC) dispersions was studied. Four isotonic buffers were used in preparing DPPC dispersions at physiological conditions for possible biological applications: (1) a standard PBS solution; (2) the above PBS with 1mM CaCl(2); (3) PBS with one tenth the previous standard phosphate salt concentrations and 2.5 mM CaCl(2); and (4) 150 mM NaCl with 2.5 mM CaCl(2) and 10mM HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid). Two protocols, with a new method and an old method (Bangham method), were used in preparing the DPPC dispersions. The DPPC dispersions prepared with the new method contained mostly vesicles and were quite stable at 25 or 37 degrees C. Dynamic light scattering (DLS) and spectroturbidimetry (ST) results showed that the DPPC vesicle sizes in buffer (4) were much smaller than those in the other buffers. When the DPPC dispersions were prepared with the new method, the diameter of the DPPC particles was smaller than those with the old method. The DPPC vesicles with the new method were more stable than those

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Author

Sook Heun Kim; Yoonjee Park; Sadis Matalon; Elias I. Franses

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 0927-7765)

Published

2008

Language

EN

Field

Materials Science (Physical Sciences)