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Plant Proteomics: Methods and Protocols (Methods in Molecular Biology Ser., Vol. 355) by Thiellement, Hervé is a nonfiction available to read on EtoBox.

What is Plant Proteomics: Methods and Protocols (Methods in Molecular Biology Ser., Vol. 355) about?

The aim of Plant Proteomics: Methods and Protocols is to present up-- date methods and protocols used by recognized scientists in the world of plant proteomics. If this world was a very small one twenty-five years ago when the first papers were published, it has since experienced exponential growth, and in most countries around the world there are laboratories working on plant proteomics. Two-dimensional gel electrophoresis is still the basic method used, but it has been improved greatly with IPG in the first dimension (Chapter 13) and with new detection methods with fluorochromes (Chapters 14 and 15). Signi- cant progress has been achieved in protein extraction, which is particularly difficult with plant tissues containing phenols, proteases, and other secondary metabolites that interfere with proteins. Standard procedures have been op- mized (Chapters 1 and 2) for peculiar tissues (Chapters 3, 4, and 5) and cellular compartments (Chapters 6 to 10). These methods rely on improvements made in the solubilization of proteins from membranes (Chapters 11 and 12). Mass spectrometry was a revolution that permitted the high throughput identifi- tion of proteins separated by 2D gels (Chapt

Who reads Plant Proteomics: Methods and Protocols (Methods in Molecular Biology Ser., Vol. 355)?

Author

Thiellement, Hervé

Publisher

Totowa, N.J.: Humana Press

Published

2007

Language

EN

ISBN

9781597452274

Category

nonfiction

Subjects

Science, Technology, Chemistry