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High-performance Liquid Chromatographic Determination of Phenylureas by Photochemically-induced Fluorescence Detection by A Muñoz de la Peña; M.C Mahedero; A Bautista-Sánchez is a Chemistry article available to read on EtoBox.

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A HPLC method, using photochemically-induced fluorescence detection, is described for the separation and determination of four phenylurea herbicides including diuron, isoproturon, linuron and neburon. A post-column photoreactor, consisting of a reactor knitted around a 4 W xenon lamp, has been included between the column and the detector, in order to transform the non-fluorescent herbicides into fluorophors. The influence of mobile phase composition, flow-rate, pH, and buffer concentration has been studied. An acetonitrile-buffer solution of potassium phosphate dibasic of pH 7 and 0.01 M concentration (60:40, v / v), was selected as optimum. For the fluorimetric detection, optimal excitation / emission wavelengths 324 / 403, 301 / 433, 335 / 411 and 326 / 385 nm were selected for the determination of diuron, isoproturon, linuron and neburon, respectively. The detection limits ranged between 0.07 and 0.46 mg / ml, according to the compound.

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Author

A Muñoz de la Peña; M.C Mahedero; A Bautista-Sánchez

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 1873-3778)

Published

2002

Language

EN

Field

Chemistry (Physical Sciences)