About this Biochemistry, Genetics and Molecular Biology article

Differential expression and biological activity of retinoic acid–induced TGFβ isoforms in embryonic palate mesenchymal cells by Paul Nugent; Linh Ma; Robert M. Greene is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

The effect of retinoic acid (RA) on TGF-b mRNA expression and protein production in murine embryonic palate mesenchymal (MEPM) cells was examined by Northern blotting and TGF-b bioassay in association with TGF-b isoform-specific neutralizing antibodies. Heat or acid activation was used to distinguish between latent and active TGF-b protein released into the culture medium. RA had little or no effect on TGF-b1 mRNA expression and protein production. In contrast, RA increased TGF-b2 and b3 protein released into the culture medium, the protein being mostly in an inactive or latent form. The amount of active TGF-b released was increased relative to the total increase in TGF-b released, suggesting that RA treatment stimulated activation of latent TGF-b. RA also increased TGF-b2 mRNA expression; we have previously shown that RA upregulates TGF-b3 mRNA in these cells. RA and TGF-b individually inhibited 3 H-thymidine incorporation into MEPM cell DNA, while, when administered simultaneously, they inhibited proliferative activity to a greater extent. Heat-or acid-activated conditioned medium (CM) from MEPM cells treated with RA was able to inhibit 3 H-thymidine incorporation into MEPM cell

Author

Paul Nugent; Linh Ma; Robert M. Greene

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0021-9541)

Published

1998

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)