About this document
Optimization of Bovine Chymosin Production by Roberta B. is a document available to read on EtoBox.
This study optimized the production and purification of recombinant bovine chymosin B in Pichia pastoris using biodiesel-derived crude glycerol as a low-cost carbon source. The research demonstrated that high cell concentration at the start of the methanol induction phase significantly increased chymosin production, achieving a specific milk-clotting activity of 6400 IMCU/mg with 95% purity. Additionally, the optimal temperature and pH for the purified chymosin
- Author
- Roberta B.
- Language
- EN