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Can I read Detection of Colorectal Cancer Cells in Peripheral Blood by Reverse-transcriptase Polymerase Chain Reaction for Cytokeratin 20 on EtoBox?
Detection of Colorectal Cancer Cells in Peripheral Blood by Reverse-transcriptase Polymerase Chain Reaction for Cytokeratin 20 by David K. Wyld; Peter Selby; Timothy J. Perren; Sonja K. Jonas; Timothy G. Allen-Mersh; John Wheeldon; Susan A. Burchill is a Medicine article available to read on EtoBox.
What is Detection of Colorectal Cancer Cells in Peripheral Blood by Reverse-transcriptase Polymerase Chain Reaction for Cytokeratin 20 about?
The staging of colorectal cancer currently depends on pathological examination of the surgical specimen and regional lymph nodes, accompanied by imaging tests such as computed tomography (CT) scanning. However, alternative molecular methods to detect circulating tumour cells in blood or bone marrow may provide additional information about the extent of disease and prognosis. We have previously reported the development of a reverse-transcriptase polymerase chain reaction (RT-PCR) for cytokeratin 20 (CK 20) mRNA to detect circulating epithelial tumour cells. In this study, we report on the application of this method for detecting circulating tumour cells in patients with colorectal cancer. Using this method, CK 20 mRNA was detected in 8/8 human colorectal cancer cell lines, in 8/9 biopsies from primary colorectal tumours and in 9/10 biopsies of liver metastasis in patients with metastatic colorectal cancer, suggesting that CK 20 may be a useful target for the detection of circulating tumour cells in this patient group. In spiking experiments, 10 cells were consistently identified in 2 ml of whole blood (1 ؋ 10 6 -1 ؋ 10 7 mononuclear cells). In 12/25 (48%) peripheral blood samples fr
Who reads Detection of Colorectal Cancer Cells in Peripheral Blood by Reverse-transcriptase Polymerase Chain Reaction for Cytokeratin 20?
It is typically read by researchers, students, and practitioners in Medicine.
- Author
- David K. Wyld; Peter Selby; Timothy J. Perren; Sonja K. Jonas; Timothy G. Allen-Mersh; John Wheeldon; Susan A. Burchill
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley; Research Square; Test accounts (ISSN 0020-7136)
- Published
- 1998
- Language
- EN
- Field
- Medicine (Health Sciences)