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Structure, linkage mapping and expression of the heart‐type fatty acid‐binding protein gene ( fabp3 ) from zebrafish ( Danio rerio ) by Rong‐Zong Liu; Eileen M. Denovan‐Wright; Jonathan M. Wright is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Structure, linkage mapping and expression of the heart‐type fatty acid‐binding protein gene ( fabp3 ) from zebrafish ( Danio rerio ) about?

We have determined the cDNA nucleotide sequence, deduced the amino acid sequence and defined the gene structure for the cellular heart‐type (H‐FABP) or fatty acid‐binding protein 3 (FABP3) from zebrafish. The zebrafish FABP3 exhibited the greatest amino acid sequence identity to fish and mammalian heart‐type FABPs. 3′ RACE and 5′ RLM‐RACE mapped two alternative polyadenylation sites and three transcription start sites, respectively. Southern blot and hybridization analysis indicated that a single __fabp3__ gene exists in the zebrafish genome. The zebrafish __fabp3__ gene consists of four exons interrupted by three introns with identical exon/intron structure and coding capacity with that of orthologous mammalian H‐FABP genes. Radiation hybrid mapping assigned the zebrafish __fabp3__ gene to linkage group 19 of the zebrafish genome. Comparative genomic analysis revealed conserved syntenies of the zebrafish __fabp3__ gene and the orthologous human and mouse __fabp3__ genes. Northern blot analysis detected an mRNA transcript of 780 nucleotides. __In situ__ hybridization of the zebrafish __fabp3__‐specific oligonucleotide probe to tissue sections of adult zebrafish revealed that the __

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Author

Rong‐Zong Liu; Eileen M. Denovan‐Wright; Jonathan M. Wright

Publisher

John Wiley and Sons; Springer; Wiley (Blackwell Publishing); Springer-Verlag; Blackwell Publishing Inc.; Springer Verlag; Wiley; Springer Science and Business Media LLC (ISSN 1432-1327)

Published

2003

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)