About this document
E. coli Competency and pUC18 Transformation by Shruti Pillai is a document available to read on EtoBox.
1. The document describes making E. coli cells competent through chemical treatment with CaCl2 and transforming them with the pUC18 vector. 2. The transformed E. coli cells are screened through double selection with ampicillin antibiotic resistance and blue/white screening using X-gal and IPTG. 3. Successful transformation is confirmed by the appearance of only white colonies, indicating insertional inactivation of the lacZ
- Author
- Shruti Pillai
- Language
- EN