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Can I read H2-Driven Reduction of Flavin by Hydrogenase Enables Cleaner Operation of Nitroreductases for Nitro-Group to Amine Reductions on EtoBox?
H2-Driven Reduction of Flavin by Hydrogenase Enables Cleaner Operation of Nitroreductases for Nitro-Group to Amine Reductions by Miguel A. Ramirez; Shiny Joseph Srinivasan; Sarah E. Cleary; Peter M. T. Todd; Holly A. Reeve; Kylie A. Vincent is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is H2-Driven Reduction of Flavin by Hydrogenase Enables Cleaner Operation of Nitroreductases for Nitro-Group to Amine Reductions about?
Hydrogenase-mediated reduction of flavin mononucleotide by H~2~ is exploited to enable cleaner application of nitroreductase enzymes for reduction of aromatic nitro functional groups. This turns the overall reaction into a biocatalytic hydrogenation. Use of flavin-containing nitroreductases in industrial biotechnology typically relies upon NADH or NADPH as reductant, together with glucose dehydrogenase and glucose as a regeneration system for the reduced nicotinamide cofactor, with 3 equivalents of the carbon-intensive glucose required for a single 6-electron nitro to amine conversion. We show here that reduced flavin mononucleotide is an alternative reductant for nitroreductases, and by combining this with H~2~-driven recycling of reduced flavin, we avoid glucose, thereby enabling atom-efficient biocatalytic nitro reductions. We compare this biocatalytic system, via green chemistry metrics, to existing strategies for biocatalytic nitro-group reductions, particularly with respect to replacing glucose with H~2~ gas. We take steps towards demonstrating industrial viability: we report an overexpression system for E. coli hydrogenase 1, giving a 12-fold improvement in enzyme yield; we
Who reads H2-Driven Reduction of Flavin by Hydrogenase Enables Cleaner Operation of Nitroreductases for Nitro-Group to Amine Reductions?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Miguel A. Ramirez; Shiny Joseph Srinivasan; Sarah E. Cleary; Peter M. T. Todd; Holly A. Reeve; Kylie A. Vincent
- Publisher
- Frontiers Media SA
- Published
- 2022
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Physical Sciences)