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Glutamate-induced Calcium Increase Mediates Magnesium Release from Mitochondria in Rat Hippocampal Neurons by Yutaka Shindo; Ai Fujimoto; Kohji Hotta; Koji Suzuki; Kotaro Oka is a Neuroscience article available to read on EtoBox.

What is Glutamate-induced Calcium Increase Mediates Magnesium Release from Mitochondria in Rat Hippocampal Neurons about?

Excess administration of glutamate is known to induce Ca 21 overload in neurons, which is the first step in excitotoxicity. Although some reports have suggested a role for Mg 21 in the excitotoxicity, little is known about its actual contribution. To investigate the role of Mg 21 in the excitotoxicity, we simultaneously measured intracellular Ca 21 and Mg 21 , using fluorescent dyes, Fura red, a fluorescent Ca 21 probe, and KMG-104, a highly selective fluorescent Mg 21 probe developed by our group, respectively. Administration of 100 lM glutamate supplemented with 10 lM glycine to rat hippocampal neurons induced an increase in intracellular Mg 21 concentration ([Mg 21 ] i ). Extracellular Mg 21 was not required for this glutamate-induced increase in [Mg 21 ] i , and no increase in intracellular Ca 21 concentration ([Ca 21 ] i ) or [Mg 21 ] i was observed in neurons in nominally Ca 21 -free medium. Application of 5 lM carbonyl cyanide p-(trifluoromethoxy) phenylhydrazone (FCCP), an uncoupler of mitochondrial inner membrane potential, also elicited increases in [Ca 21 ] i and [Mg 21 ] i . Subsequent administration of glutamate and glycine following FCCP treatment did not induce a fur

Who reads Glutamate-induced Calcium Increase Mediates Magnesium Release from Mitochondria in Rat Hippocampal Neurons?

Author

Yutaka Shindo; Ai Fujimoto; Kohji Hotta; Koji Suzuki; Kotaro Oka

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0360-4012)

Published

2010

Language

EN

Field

Neuroscience (Life Sciences)