Opening book details…
Can I read Identification of Candida auris and related species by multiplex PCR based on unique GPI protein‐encoding genes on EtoBox?
Identification of Candida auris and related species by multiplex PCR based on unique GPI protein‐encoding genes by Alvarado, María; Bartolomé Ãlvarez, Joaquín; Lockhart, Shawn R.; Valentín, Eulogio; RuizâGaitán, Alba Cecilia; Eraso, Elena; Groot, Piet W.J. de is a Medicine article available to read on EtoBox.
What is Identification of Candida auris and related species by multiplex PCR based on unique GPI protein‐encoding genes about?
## Abstract ## Background The pathogen Candida auris is rapidly gaining clinical importance because of its resistance to antifungal treatments and its persistence in hospital environments. Early and accurate diagnosis of C. auris infections is crucial, and however, the fungus has often been misidentified by commercial systems. ## Objectives To develop conventional and real‐time PCR methods for accurate and rapid identification of C. auris and its discrimination from closely related species by exploiting the uniqueness of certain glycosylphosphatidylinositol (GPI)‐modified protein‐encoding genes. ## Methods Species‐specific primers for two unique putative GPI protein‐encoding genes per species were designed for C. auris, C. haemulonii, C. pseudohaemulonii, C. duobushaemulonii, C. lusitaniae and C. albicans. Primers were blind tested for their specificity and efficiency in conventional and real‐time multiplex PCR set‐up. ## Results All primers combinations showed excellent species specificity. In multiplex mode, correct identification was aided by different‐sized amplicons for each species. Efficiency of the C. auris primers was validated using a panel of 155 C. auris isolates, inclu
Who reads Identification of Candida auris and related species by multiplex PCR based on unique GPI protein‐encoding genes?
It is typically read by researchers, students, and practitioners in Medicine.
- Author
- Alvarado, María; Bartolomé Ãlvarez, Joaquín; Lockhart, Shawn R.; Valentín, Eulogio; RuizâGaitán, Alba Cecilia; Eraso, Elena; Groot, Piet W.J. de
- Publisher
- John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0933-7407)
- Published
- 2020
- Language
- EN
- Field
- Medicine (Health Sciences)