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On the kinetics of voltage formation in purple membranes of Halobacterium salinarium by Richard W. Hendler; Lel A. Drachev; Salil Bose; Manoj K Joshi is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is On the kinetics of voltage formation in purple membranes of Halobacterium salinarium about?

The kinetics of the bacteriorhodopsin photocycle, measured by voltage changes in a closed membrane system using the direct electrometrical method (DEM) of Drachev, L.A., Jasaitus, A.A., Kaulen, A.D., Kondrashin, A.A., Liberman, E.A., Nemecek, I.B., Ostroumov, S.A., Semenov, Yu, A. & Skulachev, V.P. (1974) __Nature__**249**, 321–324 are sixfold slower than the kinetics obtained in optical studies with suspensions of purple membrane patches. In this study, we have investigated the reasons for this discrepancy. In the presence of the uncouplers carbonyl cyanide __m__‐chlorophenylhydrazone or valinomycin, the rates in the DEM system are similar to the rates in suspensions of purple membrane. Two alternative explanations for the effects of uncouplers were evaluated: (a) the ‘back‐pressure’ of the Δμ ̃~H+~ slows the kinetic steps leading to its formation, and (b) the apparent difference between the two systems is due to slow major electrogenic events that produce little or no change in optical absorbance. In the latter case, the uncouplers would decrease the __RC__ time constant for membrane capacitance leading to a quicker discharge of voltage and concomitant decrease in photocycle turn

Who reads On the kinetics of voltage formation in purple membranes of Halobacterium salinarium?

Author

Richard W. Hendler; Lel A. Drachev; Salil Bose; Manoj K Joshi

Publisher

John Wiley and Sons; Springer; Wiley (Blackwell Publishing); Springer-Verlag; Blackwell Publishing Inc.; Springer Verlag; Wiley; Springer Science and Business Media LLC (ISSN 1432-1327)

Published

2000

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)