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Study of helix–coil transition of DNA by dielectric constant measurement by Shiro Takashima is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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The thermal helix-coil transition of DNA wm ytudied by means of dielectric constant measurements. The dieIectric dispersion of native helical DNA is characterized by a. large dielectric increment and a large relaxation time, whereas that of denatured coil DNA is characterized by a small dielectric increment and a small relaxation time. The dielectric dispersion of partially denatured DNA is of particular interest. At the intermediate stage of the helix-coil transition, dispersion curves which are different from either that of helix DNA or that of coil DNA appear. This is particularly pronounced for large DNA. This indicates the presence of an intermediate form of DNA. Flow birefringence measurements were carried out simultaneously. The negative birefringence of helical DNA diminishes as the helix-coil transition proceeds. However, the extinction angle remains constant, as long as it can be measured. These results indicate the absence of intermediate forms during the helix-coil transition. The discrepancy between dielectric and birefringence measurements can be resolved by assuming that the intermediate forms are not birefringent. The size distribution of native DNA and of the indic

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Author

Shiro Takashima

Publisher

Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0006-3525)

Published

1966

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)