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Can I read LC-MS/MS method for nine different antibiotics on EtoBox?
LC-MS/MS method for nine different antibiotics by Rehm, Sophia; Rentsch, Katharina M. is a Medicine article available to read on EtoBox.
What is LC-MS/MS method for nine different antibiotics about?
## Background and aims: Tdm of antibiotics can bring benefits to patients and healthcare systems by providing better treatment and saving healthcare resources. we aimed to develop a multi-analyte method for several diverse antibiotics using lc-ms/ms. ## Materials and methods: Sample preparation consisted of protein precipitation with methanol, dilution and online extraction using a turboflow cyclone column. separation was performed on a synergi 4 μm max rp column and deuterated forms of three antibiotics were used as internal standards. ## Results: We present a lc-ms/ms method for the quantitative determination of nine antibiotics, including five cephalosporins, the carbapenem ertapenem, the fluoroquinolone ciprofloxacin as well as the combination drug trimethoprim-sulfamethoxazole from plasma. additionally, unbound ertapenem and cefazolin were analyzed in plasma water after ultrafiltration using plasma calibrators. results from routine tdm show the applicability of the method. ## Conclusion: The presented method is precise and accurate and was introduced in a university hospital, permitting fast tdm of all nine analytes. it was also used in a clinical study for measuring cefazolin
Who reads LC-MS/MS method for nine different antibiotics?
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- Author
- Rehm, Sophia; Rentsch, Katharina M.
- Publisher
- Elsevier Science; Elsevier ; Elsevier BV (ISSN 0009-8981)
- Published
- 2020
- Language
- EN
- Field
- Medicine (Health Sciences)