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Rapid visualization of proteins in isoelectric focusing gels: Isolation of brain tubulin subspecies by Kern von Hungen; Rustum C. Chin; Claude F. Baxter is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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A method for visualization of unmodified proteins in polyacrylamide isoelectric focusing (IEF) gels is described. The proteins appear as white, translucent bands when disc IEF gels are placed in water. The gel can be scanned with a scanning spectrophotometer, or the protein bands can be excised using a simple apparatus, which is described, and the protein eluted. The method is fast, selective for proteins, sensitive, and quantitative. It has been used to isolate tubulin species separated by as little as 0.5 mm in disc IEF gels.

Who reads Rapid visualization of proteins in isoelectric focusing gels: Isolation of brain tubulin subspecies?

Author

Kern von Hungen; Rustum C. Chin; Claude F. Baxter

Publisher

Elsevier Science; Elsevier ; Elsevier Inc.; Elsevier BV (ISSN 0003-2697)

Published

1983

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)