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C16 ceramide accumulates following androgen ablation in LNCaP prostate cancer cells by Masatoshi Eto; Jaafar Bennouna; Oriana C. Hunter; Pamela A. Hershberger; Tatsuya Kanto; Candace S. Johnson; Michael T. Lotze; Andrew A. Amoscato is a Medicine article available to read on EtoBox.

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## Abstract ## Background Adenocarcinoma of the prostate is the most frequently diagnosed non‐cutaneous cancer and the second leading cause of cancer‐related deaths among men in the United States. The most successful therapies to date for this tumor have involved some form of androgen ablation. However, these therapies become ineffective as the tumor evolves to an androgen‐insensitive state. Ceramide is a lipid second messenger that has been shown to mediate growth arrest or cell death when added exogenously to prostate cancer cells. As a first step toward understanding the events that lead to the transition of prostate cancer cells to an androgen‐independent state, we considered investigating the effect of androgen ablation on endogenous ceramide levels in androgen‐sensitive and androgen‐insensitive prostate cancer cells. ## Methods To investigate the mechanisms of growth arrest/apoptosis in androgen‐sensitive (LNCaP) and insensitive (DU‐145, PC–3) cells, we used various methods including nonyl acridine orange (NAO) staining, propidium iodide (PI) staining/cell‐cycle analysis, lipid analysis, and Western blotting assays. ## Results In this study, we demonstrate that androgen ablat

Who reads C16 ceramide accumulates following androgen ablation in LNCaP prostate cancer cells?

Author

Masatoshi Eto; Jaafar Bennouna; Oriana C. Hunter; Pamela A. Hershberger; Tatsuya Kanto; Candace S. Johnson; Michael T. Lotze; Andrew A. Amoscato

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0270-4137)

Published

2003

Language

EN

Field

Medicine (Health Sciences)