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Proteomic Analysis Reveals Exercise Training Induced Remodelling of Hepatokine Secretion and Uncovers Syndecan-4 as a Regulator of Hepatic Lipid Metabolism by William De Nardo; Paula M. Miotto; Jacqueline Bayliss; Shuai Nie; Stacey N. Keenan; Magdalene K. Montgomery; Matthew J. Watt is a Medicine article available to read on EtoBox.
What is Proteomic Analysis Reveals Exercise Training Induced Remodelling of Hepatokine Secretion and Uncovers Syndecan-4 as a Regulator of Hepatic Lipid Metabolism about?
## Objective Non-alcoholic fatty liver disease (NAFLD) is linked to impaired lipid metabolism and systemic insulin resistance, which is partly mediated by altered secretion of liver proteins known as hepatokines. Regular physical activity can resolve NAFLD and improve its metabolic comorbidities, however, the effects of exercise training on hepatokine secretion and the metabolic impact of exercise-regulated hepatokines in NAFLD remain unresolved. Herein, we examined the effect of endurance exercise training on hepatocyte secreted proteins with the aim of identifying proteins that regulate metabolism and reduce NAFLD severity. ## Methods C57BL/6 mice were fed a high-fat diet for six weeks to induce NAFLD. Mice were exercise trained for a further six weeks, while the control group remained sedentary. Hepatocytes were isolated two days after the last exercise bout, and intracellular and secreted proteins were detected using label-free mass spectrometry. Hepatocyte secreted factors were applied to skeletal muscle and liver __ex vivo__ and insulin action and fatty acid metabolism were assessed. Syndecan-4 (SDC4), identified as an exercise-responsive hepatokine, was overexpressed in the
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- Author
- William De Nardo; Paula M. Miotto; Jacqueline Bayliss; Shuai Nie; Stacey N. Keenan; Magdalene K. Montgomery; Matthew J. Watt
- Publisher
- Elsevier
- Published
- 2022
- Language
- EN
- Field
- Medicine (Health Sciences)