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Multi-aptamer–mediated hairpin allosteric and aptamer-assisted CRISPR system for detection of S. pneumoniae and S. aureus by Limei Zhang; Xuejing Xu; Linhong Cao; Zixin Zhu; Yinhuan Ding; Hui Jiang; Baolin Li; Jinbo Liu is a Chemistry article available to read on EtoBox.
What is Multi-aptamer–mediated hairpin allosteric and aptamer-assisted CRISPR system for detection of S. pneumoniae and S. aureus about?
A novel nucleic acid aptamer nanoprobes-mediated hairpin allosteric and aptamer-assisted CRISPR system for detection of Streptococcus pneumoniae and Staphylococcus aureus is presented. In this fluorescence assay system, utilizing the hairpin allosteric effect caused by the aptamer binding to the target bacteria, the detection of S. pneumoniae is first achieved through changes in fluorescence due to FRET. Subsequently, a Cas12a protein mixture is added to detect S. aureus. The amplified output signal is triggered by two methods to ensure the sensitivity of the method: the synergistic FRET effect is achieved by the assembly of multi-aptamer through the conjugation of streptavidin-biotin, and the trans-cleavage function of CRISPR/ Cas 12a. Under the optimized conditions, the proposed hairpin allosteric aptasensor could achieve high sensitivity (a detection limit of 135 cfu/mL) and broad-concentration quantification (dynamic range of 10 3 -10 7 cfu/mL) of S. pneumoniae. The aptamer-assisted CRISPR system for S. aureus detection showed good linearity (R 2 = 0.996) in the concentration range 10 2 -10 8 cfu/mL, with a detection limit of 39 cfu/mL. No cross-reactivity with other foodborne
Who reads Multi-aptamer–mediated hairpin allosteric and aptamer-assisted CRISPR system for detection of S. pneumoniae and S. aureus?
It is typically read by researchers, students, and practitioners in Chemistry.
- Author
- Limei Zhang; Xuejing Xu; Linhong Cao; Zixin Zhu; Yinhuan Ding; Hui Jiang; Baolin Li; Jinbo Liu
- Publisher
- Springer Science and Business Media LLC
- Published
- 2023
- Language
- EN
- Field
- Chemistry (Physical Sciences)