Can I read P2P‐R protein overexpression restricts mitotic progression at prometaphase and promotes mitotic apoptosis on EtoBox?

P2P‐R protein overexpression restricts mitotic progression at prometaphase and promotes mitotic apoptosis by Sizhi Gao; Robert E. Scott is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is P2P‐R protein overexpression restricts mitotic progression at prometaphase and promotes mitotic apoptosis about?

## Abstract Mitotic cells show a tenfold increase in immunoreactive P2P‐R protein. During mitosis, the distribution of P2P‐R protein also changes from a primary nucleolar localization in interphase cells to the periphery of chromosome in mitotic cells. These findings suggest that P2P‐R might serve a functional role in mitosis. To test this possibility, human Saos2 cells were stably transfected with P2P‐R DNA constructs and the biological effects of P2P‐R overexpression were evaluated. Overexpression of near full‐length P2P‐R was found to have paradoxical effects on the relationship between proliferation and mitosis in the nine Saos2 cell clones that were studied. A significant repression in the population doubling rates was observed in all nine clones even though a significant increase in the frequency of easily detached cells with a mitotic morphology was apparent. Flow cytometric analysis confirmed that greater than two thirds of the cells with a mitotic morphology had a 4n DNA content. Confocal microscopy further established that 85% of the mitotic cell population had prometaphase characteristics suggesting that P2P‐R overexpression restricts mitotic progression at prometaphase.

Who reads P2P‐R protein overexpression restricts mitotic progression at prometaphase and promotes mitotic apoptosis?

Author

Sizhi Gao; Robert E. Scott

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0021-9541)

Published

2002

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)