About this Biochemistry, Genetics and Molecular Biology article

Identification of proteins highly expressed in the hyphae of Candida albicans by two‐dimensional electrophoresis by Wonyoung Choi; Yung Joon Yoo; Minkyoung Kim; Duckhyang Shin; Hong Bae Jeon; Wonja Choi is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

## Abstract The increase in __Candida albicans__ infections is caused by the increase in therapies resulting in immunocompromised patients. One factor required for __C. albicans__ pathogenicity is the morphological transition from yeast to hypha. The protein profiles of whole extracts from yeasts and hyphae were examined using two‐dimensional electrophoresis to identify the proteins related to the morphological transition. Over 900 protein spots were visualized by silver staining and 11 spots were increased more than three‐fold reproducibly during hyphal differentiation. Six of the 11 spots were identified by peptide mass fingerprints, of which three represented __PRA1__, two __PHR1__ and the last __TSA1__. Vertical streak patterns of Pra1p and Phr1p indicated that post‐translational modifications seem to be caused by variable glycosylation. Comparative proteome analysis between the wild‐type and the deletion mutants, CAMB43 (__Δpra1__) and CAS10 (__Δphr1__), further confirmed the identity of __PRA1__ and __PHR1__. Interestingly, Pra1p was downregulated in __phr1__‐deleted mutants. Only __PHR1__ transcription was increased, indicating that __PRA1__ and __TSA1__ are controlled at th

Author

Wonyoung Choi; Yung Joon Yoo; Minkyoung Kim; Duckhyang Shin; Hong Bae Jeon; Wonja Choi

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); John Wiley & Sons Inc.; [Chichester]; [New York]: John Wiley & Sons, ©1985-; Wiley; Hindawi Limited (ISSN 0749-503X)

Published

2003

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)