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Expression of cation exchanger NHE and anion exchanger AE isoforms in primary human bone-derived osteoblasts by A. MOBASHERI; S. GOLDING; S. N. PAGAKIS; K. CORKEY; A. E. POCOCK; B. FERMOR; M. J. O’BRIEN; R. J. WILKINS; J. C. ELLORY; M. J. O. FRANCIS is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Expression of cation exchanger NHE and anion exchanger AE isoforms in primary human bone-derived osteoblasts about?
The authors used isoform-specific antibodies against cation (NHE) and anion (AE) exchange isoforms in order to establish their specific expression and localization in dispersed human bone-derived cells. Immunocytochemical preparations of permeabilized osteoblasts probed with polyclonal antibodies were optically analysed by conventional immunofluorescence and con-focal laser scanning microscopy. These techniques demonstrated the abundant presence of epitopes of the cation exchangers NHE1 and NHE3 and the anion exchanger AE2 in these cells. The NHE1 and NHE3 isoform proteins were predominantly located in subplasmalemmal and nucleoplasmic vesicles. The AE2 isoform was densely localized to a subcellular location characteristic of the Golgi complex. The molecular identity of the AE and NHE isoforms was investigated by RT-PCR that confirmed the presence of NHE1 and NHE3 transcripts in addition to NHE4. RT-PCR and diagnostic restriction analysis of amplified AE cDNA established preferential AE2 expression. Since AE2 has been shown to act as a sulfate transporter at low pH, it is possible that it performs this function in the osteoblast Golgi complex where sulfation reactions occur post-tr
Who reads Expression of cation exchanger NHE and anion exchanger AE isoforms in primary human bone-derived osteoblasts?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- A. MOBASHERI; S. GOLDING; S. N. PAGAKIS; K. CORKEY; A. E. POCOCK; B. FERMOR; M. J. O’BRIEN; R. J. WILKINS; J. C. ELLORY; M. J. O. FRANCIS
- Publisher
- Elsevier Science; Wiley (John Wiley & Sons); Wiley-Blackwell; Wiley (ISSN 1065-6995)
- Published
- 1999
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)