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This mini review discusses the role of protoplasts in CRISPR/Cas genome editing, highlighting their utility in validating mutagenesis efficiency and serving as a platform for DNA-free gene editing in various crops. It outlines the history, methods of protoplast isolation, regeneration, and transformation, emphasizing the challenges and advancements in protoplast technology. The review also notes the importance of developing species-specific protocols to enhance the application of protoplasts in plant breedi

Author

hichamelkhala52

Language

EN