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Fullerene Antioxidants Decrease Organophosphate-induced Acetylcholinesterase Inhibition in Vitro by Marion Ehrich; Roger Van Tassell; Yunbo Li; Zhiguo Zhou; Chris L. Kepley is a Environmental Science article available to read on EtoBox.

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Although organophosphate (OP)-induced acetylcholinesterase (AChE) inhibition is the critical mechanism causing toxicities that follow exposure, other biochemical events, including oxidative stress, have been reported to contribute to OP toxicity. Fullerenes are carbon spheres with antioxidant activity. Thus, we hypothesized that fullerenes could counteract the effects of OP compounds and tested this hypothesis using two in vitro test systems, hen brain and human neuroblastoma SH-SY5Y cells. Cells were incubated with eight different derivatized fullerene compounds before challenge with paraoxon (0=control, 5×10−8, 10−7, 2×10−7 or 5×10−7 M) or diisopropylphosphorofluoridate (DFP, 0=control, 5×10−6, 10−5, 2×10−5, and 5×10−5 M) and measurement of AChE activities. Activities of brain and SH-SY5Y AChE with OP compounds alone ranged from 55–83% lower than non-treated controls after paraoxon and from 60–92% lower than non-treated controls after DFP. Most incubations containing 1 and 10μM fullerene derivatives brought AChE activity closer to untreated controls, with improvements in AChE activity often >20%. Using dissipation of superoxide anion radicals as an indicator (xanthine oxidation a

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Author

Marion Ehrich; Roger Van Tassell; Yunbo Li; Zhiguo Zhou; Chris L. Kepley

Publisher

Elsevier BV

Published

2011

Language

EN

Field

Environmental Science (Physical Sciences)