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Characterization of Side Population Cells from Laryngeal Cancer Cell Lines by Guanglun Wan; Liang Zhou; Ming Xie; Hui Chen; Jie Tian is a Medicine article available to read on EtoBox.

What is Characterization of Side Population Cells from Laryngeal Cancer Cell Lines about?

## Abstract ## Background Analysis of the hematopoietic system has shown that bone marrow stem cells contain a subpopulation that effluxes the DNA binding dye, Hoechst 33342, out of the cell membrane. These cells are called side population (SP) cells and have been shown to have stem cell characteristics. SP cells have already been found in solid tumors, although there are no published reports examining the role of SP cells in human laryngeal cancer. ## Methods SP cells were identified in the laryngeal cancer cell lines, AMC‐HN‐8 (head and neck cancer cell line 8, from the Asan Medical Center) and Hep‐2 (human epithelial type 2) cell lines, by flow cytometry. Hep‐2 SP cells were isolated and analyzed in proliferation assays, differentiation studies, radiotherapy resistance studies, and tumorigenesis ability. ## Results The AMC‐HN‐8 and Hep‐2 cell lines contained 11.8 ± 1.7% and 17.1 ± 2.0% SP cells, respectively. SP cell proportion was reduced by verapamil. Hep‐2 SP cells exhibited higher self‐regeneration, proliferation, radiotherapy resistance, and tumorigenicity. ## Conclusions SP cells purified from the Hep‐2 cell line harbor cancer stem cell–like properties, and they are hetero

Who reads Characterization of Side Population Cells from Laryngeal Cancer Cell Lines?

Author

Guanglun Wan; Liang Zhou; Ming Xie; Hui Chen; Jie Tian

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley; Research Square (ISSN 1043-3074)

Published

2010

Language

EN

Field

Medicine (Health Sciences)