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Toxoplasma gondii : Preventive and therapeutic effects of morphine and evaluation of treatment parameters of tachyzoites and infected macrophages in vitro and in a murine model by Leila Zaki; Fatemeh Ghaffarifar; Zohreh Sharifi; John Horton; Javid Sadraei is a scholarly article available to read on EtoBox.

What is Toxoplasma gondii : Preventive and therapeutic effects of morphine and evaluation of treatment parameters of tachyzoites and infected macrophages in vitro and in a murine model about?

Common medicines for the treatment of toxoplasmosis have limited efficacy and unwanted side effects. Opiates can effect both innate and cell-mediated immunity and stimulate the immune responses in different parasitic infections. In this work, preventive and therapeutic effects of morphine were evaluated on the tachyzoites of __Toxoplasma gondii__ and infected macrophages __in vitro__ and in a murine model. Different concentrations of morphine (0.1 and 0.01 μg/ml) were evaluated on mortality rate of __T. gondii__ by direct counting after 3 and 24 hours. The cytotoxic and apoptotic effects of these drugs were measured by the MTT assays and flow cytometry analysis, respectively. The same procedures were assessed in __T. gondii__-infected macrophages. The parasite loads were determined using quantitative polymerase chain reaction (qPCR). For in __vivo__ assessment, BALB/c mice treated with morphine before or after infection with tachyzoites. The survival rate of animals, parasite load in the spleen, and the IFN-γ and IL-4 cytokines levels were measured. Morphine was effective on tachyzoites of __T. gondii__ and had a reverse relationship with its concentration. The results of flow cyto

Author

Leila Zaki; Fatemeh Ghaffarifar; Zohreh Sharifi; John Horton; Javid Sadraei

Published

2020

Language

EN