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Two-dimensional Nanosecond Electric Field Mapping Based on Cell Electropermeabilization by Meng-Tse Chen; Chunqi Jiang; P Thomas Vernier; Yu-Hsuan Wu; Martin A Gundersen is a scholarly article available to read on EtoBox.

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Nanosecond, megavolt-per-meter electric pulses cause permeabilization of cells to small molecules, programmed cell death (apoptosis) in tumor cells, and are under evaluation as a treatment for skin cancer. We use nanoelectroporation and fluorescence imaging to construct two-dimensional maps of the electric field associated with delivery of 15 ns, 10 kV pulses to monolayers of the human prostate cancer cell line PC3 from three different electrode configurations: single-needle, five-needle, and flat-cut coaxial cable. Influx of the normally impermeant fluorescent dye YO-PRO-1 serves as a sensitive indicator of membrane permeabilization. The level of fluorescence emission after pulse exposure is proportional to the applied electric field strength. Spatial electric field distributions were compared in a plane normal to the center axis and 15-20 mum from the tip of the center electrode. Measurement results agree well with models for the three electrode arrangements evaluated in this study. This live-cell method for measuring a nanosecond pulsed electric field distribution provides an operationally meaningful calibration of electrode designs for biological applications and permits visual

Author

Meng-Tse Chen; Chunqi Jiang; P Thomas Vernier; Yu-Hsuan Wu; Martin A Gundersen

Publisher

Springer (Biomed Central Ltd.); BioMed Central; London: PhysMath Central, 2008-2010.; Springer Science and Business Media LLC (ISSN 1757-5036)

Published

2009

Language

EN