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Metabolic profiling of transgenic adenocarcinoma of mouse prostate (TRAMP) Tissue by ^1^H‐NMR analysis: evidence for unusual phospholipid metabolism by Friederike Teichert; Richard D. Verschoyle; Peter Greaves; Richard E. Edwards; Orla Teahan; Donald J.L. Jones; Ian D. Wilson; Peter B. Farmer; William P. Steward; Timothy W. Gant; Andreas J. Gescher; Hector C. Keun is a Medicine article available to read on EtoBox.
What is Metabolic profiling of transgenic adenocarcinoma of mouse prostate (TRAMP) Tissue by ^1^H‐NMR analysis: evidence for unusual phospholipid metabolism about?
## Abstract ## BACKGROUND The TRansgenic Adenocarcinoma of the Mouse Prostate (TRAMP) mouse model has frequently been used in preclinical studies with chemotherapeutic/chemopreventive rationales. Here the hypothesis was tested using ^1^H‐NMR‐based metabolic profiling that the TRAMP tumor metabolic phenotype resembles that reported for human prostate cancer. ## METHODS Aqueous extracts or intact tissues of normal prostate from 8‐ (“young”) or 28‐(“old”) week‐old C57BL/6J wild‐type mice or of prostate tumor from age‐matched TRAMP mice were analyzed by ^1^H‐NMR. Results were compared with immunohistochemical findings. Expression of choline kinase was studied at the protein and mRNA levels. ## RESULTS In young TRAMP mice presenting with zonal hyperplasia, the ratio of glycerophosphocholine (GPC) to phosphocholine (PC) was 22% below that in wild‐type mice (__P__ < 0.05). In old TRAMP mice with well‐defined malignancy, reduced tumor levels of citrate (49%), choline (33%), PC (57%), GPC (66%), and glycerophosphoinositol (61%) were observed relative to normal prostate (__P__ < 0.05). Hierarchical cluster analysis of metabolite levels distinguished between normal and malignant tissue in old
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- Author
- Friederike Teichert; Richard D. Verschoyle; Peter Greaves; Richard E. Edwards; Orla Teahan; Donald J.L. Jones; Ian D. Wilson; Peter B. Farmer; William P. Steward; Timothy W. Gant; Andreas J. Gescher; Hector C. Keun
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0270-4137)
- Published
- 2008
- Language
- EN
- Field
- Medicine (Health Sciences)