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ADAMTS‐5 and Intervertebral Disc Degeneration: The Results of Tissue Immunohistochemistry and In Vitro Cell Culture by Chang‐Qing Zhao; Yue‐Hui Zhang; Sheng‐Dan Jiang; Hai Li; Lei‐Sheng Jiang; Li‐Yang Dai is a Medicine article available to read on EtoBox.
What is ADAMTS‐5 and Intervertebral Disc Degeneration: The Results of Tissue Immunohistochemistry and In Vitro Cell Culture about?
## Abstract Matrix metalloproteinases (MMPs) are known to be involved in IVD degeneration by hydrolyzing the extracellular matrix (ECM), especially the collagens. The degradation of proteoglycans, which is another main ECM component in the IVD, however, has not been extensively investigated. This study aimed to determine the expression of ADAMTS‐5 in human herniated intervertebral disc (IVD) tissues and to investigate whether interleukin‐1β (IL‐1β)‐induced expression of ADAMTS‐5 is mediated by nitric oxide (NO). Forty‐five herniated IVDs were harvested and immunostained to determine the distribution and type of ADAMTS‐5 expressing cells. Rat NP cells maintained in alginate beads were treated with IL‐1β, accumulation of NO was detected by Griess reaction, the expression of ADAMTS‐5 and inducible nitric oxide synthase (iNOS) was analyzed by reverse transcriptase‐polymerase chain reaction (RT‐PCR), the content of proteoglycans in alginate beads was visualized by alcian blue staining, and the effect of aminoguanidine on the changes in alginate beads induced by IL‐1β treatment were also examined. Immunohistochemical results from 45 herniated discs showed that ADAMTS‐5‐positive cells are
Who reads ADAMTS‐5 and Intervertebral Disc Degeneration: The Results of Tissue Immunohistochemistry and In Vitro Cell Culture?
It is typically read by researchers, students, and practitioners in Medicine.
- Author
- Chang‐Qing Zhao; Yue‐Hui Zhang; Sheng‐Dan Jiang; Hai Li; Lei‐Sheng Jiang; Li‐Yang Dai
- Publisher
- Elsevier Science; Wiley (John Wiley & Sons); Wiley-Blackwell; Wiley (ISSN 0736-0266)
- Published
- 2010
- Language
- EN
- Field
- Medicine (Health Sciences)