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P3489Endothelial adenosine-to-inosine RNA editing is indispensable for vascular integrity by Tual-Chalot, S; Bonini, F; Vlachogiannis, N I; Doddaballapur, A; Shook, K; Gatsiou, A; Stellos, K is a Medicine article available to read on EtoBox.

What is P3489Endothelial adenosine-to-inosine RNA editing is indispensable for vascular integrity about?

## Abstract ## Background Adenosine (A)-to-inosine (I) RNA editing is a powerful upstream regulator of double-stranded RNA (dsRNA) metabolism, its role however in tissue homeostasis remains poorly understood. We have recently reported that RNA editing is dysregulated in human atherosclerotic heart disease, although its role in the maintenance of endothelial cell (EC) function is unknown. ## Purpose The goal of the present study is to evaluate the role of EC-specific ADAR1 in vascular homeostasis in vivo. ## Methods Constitutional or inducible EC restricted ADAR1 ablation was achieved by crossing mice carrying a conditional (floxed) ADAR1 allele with either a Tie2-Cre or with a tamoxifen-inducible Cdh5-CreERT2 mouse line. EC function was assessed by the postnatal retinal angiogenesis model and barrier function assays and immunohistochemistry of lung and liver tissues. Cell culture assays, gene-silencing techniques, RT-qPCR, western blot and confocal microscopy were utilized to assess the ADAR1 effects in intracellular endothelial signalling. ## Results Constitutive endothelial ADAR1 ablation caused prenatal lethality at embryonic stage E13.5, demonstrating an essential role for endo

Who reads P3489Endothelial adenosine-to-inosine RNA editing is indispensable for vascular integrity?

Author

Tual-Chalot, S; Bonini, F; Vlachogiannis, N I; Doddaballapur, A; Shook, K; Gatsiou, A; Stellos, K

Publisher

Oxford University Press; Oxford University Press (OUP); Elsevier BV (ISSN 0195-668X)

Published

2019

Language

EN

Field

Medicine (Health Sciences)