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Down‐regulation of CYP1A2 induction during the maturation of mouse cerebellar granule cells in culture: role of nitric oxide accumulation by Sonia Mulero‐Navarro; Belen Santiago‐Josefat; Eulalia Pozo‐Guisado; Jaime M. Merino; Pedro M. Fernandez‐Salguero is a Neuroscience article available to read on EtoBox.
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## Abstract Nitric oxide (NO) is responsible for cytochrome P450 (CYP450) loss during isolation and cytokine treatment of primary rat hepatocytes. As P450s mediate the metabolism of toxic chemicals, their inhibition could compromise the cells competence to eliminate toxins, a condition potentially relevant in neurological diseases involving constitutive activation of nitric oxide synthase (NOS) and NO over‐production. Here, we have investigated the correlation between NO accumulation and CYP1A2 down‐regulation during maturation of mouse cerebellar granule cells (CGC). As neurons matured in culture, the inducible levels of CYP1A2 protein and catalytic activity decreased to almost undetectable values. In parallel, a significant increase in NO concentration was observed. Neuronal NOS remained constitutively active during maturation, thus contributing to NO accumulation. The NOS inhibitor l‐NAME, restored CYP1A2 catalytic activity up to 9 days __in vitro__, supporting a role for NO in the inhibition process. Maturation was also followed by increased NMDA receptor activity and intracellular Ca^2+^ concentration. We suggest that maintained NOS activity during CGC maturation could lead to
Who reads Down‐regulation of CYP1A2 induction during the maturation of mouse cerebellar granule cells in culture: role of nitric oxide accumulation?
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- Author
- Sonia Mulero‐Navarro; Belen Santiago‐Josefat; Eulalia Pozo‐Guisado; Jaime M. Merino; Pedro M. Fernandez‐Salguero
- Publisher
- John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0953-816X)
- Published
- 2003
- Language
- EN
- Field
- Neuroscience (Life Sciences)