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Can I read Analysis of dendritic cell trafficking using EGFP-transgenic mice on EtoBox?
Analysis of dendritic cell trafficking using EGFP-transgenic mice by Andreas A.O. Eggert; Robbert van der Voort; Ruurd Torensma; Veronique Moulin; Otto C. Boerman; Wim J.G. Oyen; C.J.A. Punt; Heleen Diepstra; Annemiek J. de Boer; Carl G. Figdor; Gosse J. Adema is a Immunology and Microbiology article available to read on EtoBox.
What is Analysis of dendritic cell trafficking using EGFP-transgenic mice about?
Dendritic cells (DCs) are professional antigen presenting cells, well equipped to initiate an immune response. For effective induction of an immune response, DC should migrate from the periphery to the lymph node to present the antigen to T lymphocytes. Currently, tumor-antigen loaded DCs are used in clinical vaccination trials in cancer patients. To investigate the migratory capacity of DC in vivo, a variety of fluorescent and radioactive labels have been used. Here we introduce a novel tool to study DC migration in vivo: DCs generated from enhanced green fluorescent protein (EGFP)-transgenic mice. DC from EGFPtransgenic mice display typical DC behavior and can be matured without affecting their autofluorescence in vitro. In addition, the continuously produced cytoplasmic EGFP in living cells functions as a viability marker, since EGFP released from dying cells does not stain DC from C57Bl/6 mice upon coculture. In vivo migration studies using EGFP-DC and indium-111-labeled DC were performed to determine the efficiency of i.d. versus s.c. administered DC to reach the draining lymph node. The analysis demonstrates that i.d. injection increases the amount of EGFP-DC/indium-111-label
Who reads Analysis of dendritic cell trafficking using EGFP-transgenic mice?
It is typically read by researchers, students, and practitioners in Immunology and Microbiology.
- Author
- Andreas A.O. Eggert; Robbert van der Voort; Ruurd Torensma; Veronique Moulin; Otto C. Boerman; Wim J.G. Oyen; C.J.A. Punt; Heleen Diepstra; Annemiek J. de Boer; Carl G. Figdor; Gosse J. Adema
- Publisher
- Elsevier Science; Elsevier ; Elsevier BV (ISSN 0165-2478)
- Published
- 2003
- Language
- EN
- Field
- Immunology and Microbiology (Life Sciences)