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Monoclonal antibodies to sperm surface antigens of the brown alga Fucus serratus exhibit region-, gamete-, species- and genus-preferential binding by J. L. Jones; J. A. Callow; J. R. Green is a Agricultural and Biological Sciences article available to read on EtoBox.

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A panel of twelve monoclonal antibodies (MAbs), designated FSI to FS12, have been raised against surface antigens of Fucus serratus sperm. The antibodies were selected on the basis that they show region-, gamete-, species-or genus-preferential binding. Indirect immunofluorescence shows that the antigens bound by the MAbs are distributed non-randomly over the cell surface. Seven MAbs (FS1, FS3, FS4, FS6, FS8, FS9, FSI0) bind antigens located primarily on the cell body, while the others (FS2, FS5, FS7, FSI1, FS12) bind antigens located primarily on the anterior flagellum. Of the MAbs that label the anterior flagellum, FS2, FS5, FS7 and FS12 form a 'halo' at the perimeter of the flagellum. Electron microscopic-immunogold studies indicate that the 'halo' results from labelling of the mastigonemes, as opposed to the flagellar plasmamembrane. Gamete-preferential binding of antibodies was detected using an enzyme-linked immunosorbent assay with egg membrane vesicles. Eight of the MAbs bind sperm antigens not common to eggs, though FS2, FS4, FS5 and FS9 bind antigens present on both sperm and eggs. In studies of species-and genus-specificity FS2, FS3, FS5, FS6, FS7, FS8, FSI0, FSll and FS1

Who reads Monoclonal antibodies to sperm surface antigens of the brown alga Fucus serratus exhibit region-, gamete-, species- and genus-preferential binding?

Author

J. L. Jones; J. A. Callow; J. R. Green

Publisher

Springer-Verlag; Springer Verlag; Springer Science and Business Media LLC; Society for Mining, Metallurgy and Exploration Inc. (ISSN 0032-0935)

Published

1988

Language

EN

Field

Agricultural and Biological Sciences (Life Sciences)