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What is Nematode PCR Primers for 18S rRNA about?

This document describes the design of new nematode-specific PCR primers for amplifying the 18S small subunit rRNA gene from nematodes. The primers were designed based on an alignment of nematode 18S sequences that identified regions conserved among nematodes but not present in other groups like fungi. Testing of the primers showed that a forward primer (Nem_18S_F) and reverse primer (Nem_18S_R) reliably amplified a ~1kb fragment specifically from nematode DNA. The primers and PCR conditions allow researcher

Author

Fernanda g

Language

EN