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Pim-1 kinase phosphorylates RUNX family transcription factors and enhances their activity by Teija LT Aho; Jouko Sandholm; Katriina J Peltola; Yoshiaki Ito; Päivi J Koskinen is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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## Background The __pim__ family genes encode oncogenic serine/threonine kinases which in hematopoietic cells have been implicated in cytokine-dependent signaling as well as in lymphomagenesis, especially in cooperation with other oncogenes such as __myc__, __bcl__-2 or __Runx__ family genes. The __Runx__ genes encode α-subunits of heterodimeric transcription factors which regulate cell proliferation and differentiation in various tissues during development and which can become leukemogenic upon aberrant expression. ## Results Here we have identified novel protein-protein interactions between the Pim-1 kinase and the RUNX family transcription factors. Using the yeast two-hybrid system, we were able to show that the C-terminal part of human RUNX3 associates with Pim-1. This result was confirmed in cell culture, where full-length murine Runx1 and Runx3 both coprecipitated and colocalized with Pim-1. Furthermore, catalytically active Pim-1 kinase was able to phosphorylate Runx1 and Runx3 proteins and enhance the transactivation activity of Runx1 in a dose-dependent fashion. ## Conclusion Altogether, our results suggest that mammalian RUNX family transcription factors are novel binding

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Author

Teija LT Aho; Jouko Sandholm; Katriina J Peltola; Yoshiaki Ito; Päivi J Koskinen

Publisher

BioMed Central; Springer (Biomed Central Ltd.); London: BioMed Central, [2000]-2018.; Springer Science and Business Media LLC (ISSN 1471-2121)

Published

2006

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)