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This document describes optimization of the use of phosphomolybdic acid (PMA) staining for quantification of cholesterol and selected bile acids separated by thin layer chromatography. Key parameters studied included PMA concentration, heating temperature and time. The best conditions found for sensitive detection were heating separated samples spotted on silica gel or octadecylsilane plates at 50-80°C for at least 20 minutes after spraying with 10% PMA in methanol. This provided more robust and sensitive q

Author

Jerika Marie Arceo

Language

EN