Can I read Biogenesis and release of endothelial extracellular vesicles: Morphological aspects on EtoBox?

Biogenesis and release of endothelial extracellular vesicles: Morphological aspects by Clara Elsner; Süleyman Ergün; Nicole Wagner is a Medicine article available to read on EtoBox.

What is Biogenesis and release of endothelial extracellular vesicles: Morphological aspects about?

Background Cell-cell communication through extracellular vesicles (EVs) including exosomes, microvesicles and apoptotic bodies has been shown to be important in physiological homoeostasis as well as pathological processes such as atherosclerosis. However, while the cellular machinery controlling EV formation and composition has been studied during the past decade, less is known about the morphological process of their formation and release. Methods Using different electron microscopic approaches including transmission-, scanning-, immun-, and serial block face electron microscopy we studied the morphogenetic events of EV formation and release. We analysed the different steps of EV formation and release in cultured myocardial endothelial (MyEnd) and aortic endothelial (AoEnd) cell lines under serum starvation and under inflammatory conditions. Results We show that in a narrow time frame, the number of active cells and microvesicle (MV) producing cells increased in dependence of time spent in cultivation and additional stimulation by TNF-α. However, MV secretion was a highly heterogeneous process which couldn ́t be seen in all cells cultivated under the same conditions. Release of MV

Who reads Biogenesis and release of endothelial extracellular vesicles: Morphological aspects?

Author

Clara Elsner; Süleyman Ergün; Nicole Wagner

Publisher

Elsevier BV

Published

2023

Language

EN

Field

Medicine (Health Sciences)