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Developmental Abnormalities in Rat Embryos Leading to Tibial Ray Deficiencies Induced by Busulfan by Miwako Otsuji; Masatoshi Takahara; Takuji Naruse; Dehong Guan; Mikio Harada; Piao Zhe; Michiaki Takagi; Toshihiko Ogino is a Medicine article available to read on EtoBox.

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## Abstract ## BACKGROUND Little is known about the developmental changes associated with tibial ray deficiencies. The aim of this study was to detect cell death, proliferation, and gene expression that result in tibial ray deficiencies. ## METHODS We induced tibial ray deficiencies in rat embryos using a teratogenic agent (busulfan) and observed the developmental changes in 1126 hindlimbs. We performed Nile blue staining, whole mount in situ hybridization for fibroblast growth factor 8 (__Fgf8__), bone morphogenetic protein 4 (__Bmp4__) and Sonic hedgehog (__Shh__), terminal deoxynucleotidyl transferase‐mediated dUTP‐biotin nick end‐labeling (TUNEL) and assessment of cell proliferation by 5‐bromo‐2′‐deoxy‐uridine (BrdU)/anti‐BrdU immunohistochemistry. ## RESULTS In situ hybridization showed reductions in __Fgf8__ and __Bmp4__ expression. Histological examination showed a delay of mesenchymal condensation, increased mesenchymal cell death, decreased mesenchymal cell proliferation, and a reduction in the number of mesenchymal cells. These abnormalities may cause hypoplasia of the limb. __Bmp4__ expression was markedly reduced in the anterior mesenchyme. __Shh__ was expressed in the

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Author

Miwako Otsuji; Masatoshi Takahara; Takuji Naruse; Dehong Guan; Mikio Harada; Piao Zhe; Michiaki Takagi; Toshihiko Ogino

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 1542-0752)

Published

2005

Language

EN

Field

Medicine (Health Sciences)