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The Primary Antibody Repertoire of κ‐Deficient Mice is Characterized by Non‐Stochastic V~λ~1 + V~H~ Gene Family Pairings and a Higher Degree of Self‐Reactivity by Knott; Bona; Kaushik is a Immunology and Microbiology article available to read on EtoBox.

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We have investigated the primary antibody repertoire of genetically manipulated 129/Sv κ‐deficient (JC~κ~D) mice, in order to understand the contributions of the λ‐light chain, in the absence of an otherwise predominant κ‐light chain, to the development of humoral immunity. The expression of V~λ~1 gene (λ~1~ and λ~3~ subtypes) and the V~λ~1 + V~H~ (J558, 36–60, V~H~11 and S107) gene family associations were studied in 7.43 × 10^3^ mitogen‐activated splenic B‐lymphocyte clones of JC~κ~D origin. Furthermore, the functional significance of the exclusive expression of the λ‐light chain, in the peripheral B‐cell repertoire of JC~κ~D mice, was analysed by determining natural autoantibody specificities in the circulating serum immunoglobulin and the frequency of autoreactive B‐lymphocyte clones in the peripheral B‐lymphocyte repertoire. These experiments revealed that: first, of the three available V~λ~ genes at the λ locus, the V~λ~1 gene is the one that is expressed most frequently (59.9%); second, non‐random V~λ~1 + V~H~ (J558, 36–60) gene family pairings occur in κ‐deficient mice; and third, a higher degree of self‐reactivity is generated as a result of exclusive use of the λ‐light ch

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Author

Knott; Bona; Kaushik

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0300-9475)

Published

1998

Language

EN

Field

Immunology and Microbiology (Life Sciences)