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Ca^2+^ store depletion and endoplasmic reticulum stress are involved in P2X7 receptor‐mediated neurotoxicity in differentiated NG108‐15 cells by Chia-Chia Chao; Chieh-Chen Huang; Dah-Yuu Lu; Kar-Lok Wong; Yun-Ru Chen; Tzu-Hurng Cheng; Yuk-Man Leung is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
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## Abstract P2X7 receptor (P2X7R) activation by extracellular ATP triggers influx of Na^+^ and Ca^2+^, cytosolic Ca^2+^ overload and consequently cytotoxicity. Whether disturbances in endoplasmic reticulum (ER) Ca^2+^ homeostasis and ER stress are involved in P2X7R‐mediated cell death is unknown. In this study, a P2X7R agonist (BzATP) was used to activate P2X7R in differentiated NG108‐15 neuronal cells. In a concentration‐dependent manner, application of BzATP (10–100 μM) immediately raised cytosolic Ca^2+^ concentration ([Ca^2+^]~i~) and caused cell death after a 24‐h incubation. P2X7R activation for 2 h did not cause cell death but resulted in a sustained reduction in ER Ca^2+^ pool size, as evidenced by a diminished cyclopiazonic acid‐induced Ca^2+^ discharge (fura 2 assay) and a lower fluorescent signal in cells loaded with Mag‐fura 2 (ER‐specific Ca^2+^‐fluorescent dye). Furthermore, P2X7R activation (2 h) led to the appearance of markers of ER stress [phosphorylated α subunit of eukaryotic initiation factor 2 (p‐eIF2α) and C/EBP homologous protein (CHOP)] and apoptosis (cleaved caspase 3). Xestospongin C (XeC), an antagonist of inositol‐1,4,5‐trisphosphate (IP~3~) receptor (I
Who reads Ca^2+^ store depletion and endoplasmic reticulum stress are involved in P2X7 receptor‐mediated neurotoxicity in differentiated NG108‐15 cells?
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- Author
- Chia-Chia Chao; Chieh-Chen Huang; Dah-Yuu Lu; Kar-Lok Wong; Yun-Ru Chen; Tzu-Hurng Cheng; Yuk-Man Leung
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0730-2312)
- Published
- 2012
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)