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The virulence of Salmonella Enteritidis in Galleria mellonella is improved by N-dodecanoyl-homoserine lactone by Leonardo Luiz de Freitas; Fernanda Pereira da Silva; Kenner Morais Fernandes; Deisy Guimarães Carneiro; Leandro Licursi de Oliveira; Gustavo Ferreira Martins; Maria Cristina Dantas Vanetti is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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Salmonella is a food and waterborne pathogen responsible for outbreaks worldwide, and it can survive during passage through the stomach and inside host phagocytic cells. Virulence genes are required for infection and survival in macrophages, and some are under the regulation of the quorum sensing (QS) system. This study investigated the influence of the autoinducer 1 (AI-1), N-dodecanoyl-homoserine lactone (C12-HSL), on the virulence of Salmonella PT4 using Galleria mellonella as an infection model. Salmonella PT4 was grown in the presence and absence of C12-HSL under anaerobic conditions for 7 h, and the expression of rpoS, arcA, arcB, and invA genes was evaluated. After the inoculation of G. mellonella with the median lethal dose (LD) of Salmonella PT4, the survival of bacteria inside the larvae and their health status (health index scoring) were monitored, as well as the pigment, nitric oxide (NO), superoxide dismutase (SOD), and catalase (CAT) production. Also, the hemocyte viability, the induction of caspase-3, and microtubule-associated light chain 3 (LC3) protein in hemocytes were evaluated. Salmonella PT4 growing in the presence of C12-HSL showed increased rpoS, arcA, arcB,

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Author

Leonardo Luiz de Freitas; Fernanda Pereira da Silva; Kenner Morais Fernandes; Deisy Guimarães Carneiro; Leandro Licursi de Oliveira; Gustavo Ferreira Martins; Maria Cristina Dantas Vanetti

Publisher

Elsevier BV

Published

2021

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)