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Analyses of Secondary Structures in DNA by Pyrosequencing by Mostafa Ronaghi; Malin Nygren; Joakim Lundeberg; Pål Nyrén is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Analyses of Secondary Structures in DNA by Pyrosequencing about?
A common problem in conventional DNA sequencing is the occurrence of DNA sequence compressions during gel electrophoresis, leading to misreading of the sequence. These compressions are usually due to secondary structures in the DNA fragment. In this study, we present a non-gel-based DNA sequencing technique that facilitates analysis of such DNA regions. A part of the polymorphic pertussis toxin promoter region in five different Bordetella species was successfully resolved by the new technique. The obtained sequence data revealed four related palindromic sequences. The ability of different DNA polymerases to read through such secondary structures is also described.
Who reads Analyses of Secondary Structures in DNA by Pyrosequencing?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Mostafa Ronaghi; Malin Nygren; Joakim Lundeberg; Pål Nyrén
- Publisher
- Elsevier Science; Elsevier ; Elsevier Inc.; Elsevier BV (ISSN 0003-2697)
- Published
- 1999
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)