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Biochemical, genetic and molecular characterization of new respiratory-deficient mutants inChlamydomonas reinhardtii by Marie-Pierre Dorthu; Suzanne Remy; Marie-Rose Michel-Wolwertz; Laurence Colleaux; Didier Breyer; Marie-Claire Beckers; Serge Englebert; Claire Duyckaerts; Francis E. Sluse; René F. Matagne is a Agricultural and Biological Sciences article available to read on EtoBox.

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Eight respiratory-deficient mutants of Chlamydomonas reinhardtii have been isolated after mutagenic treatment with acriflavine or ethidium bromide. They are characterized by their inability to grow or their very reduced growth under heterotrophic conditions. One mutation (Class III) is of nuclear origin whereas the seven remaining mutants (Classes I and II) display a predominantly paternal mt- inheritance, typical of mutations residing in the mitochondrial DNA. Biochemical analysis has shown that all mutants are deficient in the cyanide-sensitive cytochrome pathway of the respiration whereas the alternative pathway is still functional. Measurements of complexes II + III (antimycin-sensitive succinate-cytochrome c oxido-reductase) and complex IV (cytochrome c oxidase) activities allowed to conclude that six mutations have to be localized in the mitochondrial apocytochrome b (COB) gene, one in the mitochondrial cytochrome oxidase subunit I (COI) gene and one in a nuclear gene encoding a component of the cytochrome oxidase complex. By using specific probes, we have moreover demonstrated that five mutants (Class II mutants) contain mitochondrial DNA molecules deleted in the terminal en

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Author

Marie-Pierre Dorthu; Suzanne Remy; Marie-Rose Michel-Wolwertz; Laurence Colleaux; Didier Breyer; Marie-Claire Beckers; Serge Englebert; Claire Duyckaerts; Francis E. Sluse; René F. Matagne

Publisher

Springer; Springer-Verlag; Kluwer Academic Publishers; Springer Science and Business Media LLC; Society for Mining, Metallurgy and Exploration Inc. (ISSN 0167-4412)

Published

1992

Language

EN

Field

Agricultural and Biological Sciences (Life Sciences)