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What is Enzymatic Assay for Polygalacturonase about?
This document describes an enzymatic assay to measure polygalacturonase activity. It involves incubating polygalacturonic acid with the enzyme, then using copper and arsenomolybdate reagents to detect reducing sugars produced, quantified using a spectrophotometer. A standard curve with D-galacturonic acid is used to calculate enzyme activity in units defined as micromoles of reducing sugars released per minute. The final reaction contains 0.5% polygalacturonic acid substrate and 0.002-0.1 units of polygalac
- Author
- chaima
- Language
- EN