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A trans-kingdom T6SS effector induces the fragmentation of the mitochondrial network and activates innate immune receptor NLRX1 to promote infection by Sá-Pessoa, Joana (author);López-Montesino, Sara (author);Przybyszewska, Kornelia (author);Rodríguez-Escudero, Isabel (author);Marshall, Helina (author);Ova, Adelia (author);Schroeder, Gunnar N. (author);Barabas, Peter (author);Molina, María (author);Curtis, Tim (author);Cid, Víctor J. (author);Bengoechea, José A. (author) is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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## Abstract Bacteria can inhibit the growth of other bacteria by injecting effectors using a type VI secretion system (T6SS). T6SS effectors can also be injected into eukaryotic cells to facilitate bacterial survival, often by targeting the cytoskeleton. Here, we show that the trans-kingdom antimicrobial T6SS effector VgrG4 from Klebsiella pneumoniae triggers the fragmentation of the mitochondrial network. VgrG4 colocalizes with the endoplasmic reticulum (ER) protein mitofusin 2. VgrG4 induces the transfer of Ca^2+^ from the ER to the mitochondria, activating Drp1 (a regulator of mitochondrial fission) thus leading to mitochondrial network fragmentation. Ca^2+^ elevation also induces the activation of the innate immunity receptor NLRX1 to produce reactive oxygen species (ROS). NLRX1-induced ROS limits NF-κB activation by modulating the degradation of the NF-κB inhibitor IκBα. The degradation of IκBα is triggered by the ubiquitin ligase SCF^β-TrCP^, which requires the modification of the cullin-1 subunit by NEDD8. VgrG4 abrogates the NEDDylation of cullin-1 by inactivation of Ubc12, the NEDD8-conjugating enzyme. Our work provides an example of T6SS manipulation of eukaryotic cells v

Who reads A trans-kingdom T6SS effector induces the fragmentation of the mitochondrial network and activates innate immune receptor NLRX1 to promote infection?

Author

Sá-Pessoa, Joana (author);López-Montesino, Sara (author);Przybyszewska, Kornelia (author);Rodríguez-Escudero, Isabel (author);Marshall, Helina (author);Ova, Adelia (author);Schroeder, Gunnar N. (author);Barabas, Peter (author);Molina, María (author);Curtis, Tim (author);Cid, Víctor J. (author);Bengoechea, José A. (author)

Publisher

Springer Science and Business Media LLC

Published

2023

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)