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Determination of Tiodazosin in Plasma and Whole Blood by High-performance Liquid Chromatography by Bruce A. Mico; Robert A. Baughman Jr.; Leslie Z. Benet is a Chemistry article available to read on EtoBox.

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Tiodazosin levuhnate, a chemical and pharmacological analogue of prazosin (Minipress, Pfizer), is a new antihypertensive agent currently in clinical trials. The structures for these two drugs are indicated below. PRAZOSIN NH2 TIODAZOSIN Although there are no published assays of tiodazosin, the assay procedure currently in use at Bristol Laboratories [I] is based on the procedure for prazosin analysis developed by Twomey and Hobbs 121. This technique involves extraction of the alkaline sample with ethyl acetate, followed by back extraction with a sulfuric acid solution. After a second alkaline extraction with ethyl acetate, the organic layer is evaporated to-dryness with a stream of nitrogen gas, then reconstituted with the mobile phase and analyzed by high-performance liquid chromatography (DPLC). This procedure is lengthy and requires 2-4 ml of biological sample to obtain the desired sensitivity\_ We report here a method for the determination of tiodazosin in plasma and whole blood which is based on our previously published assay of prazosin [3]. The method is simple, reliable and sensitive; it involves no extraction steps and requires only 0.2 ml of biological sample.

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Author

Bruce A. Mico; Robert A. Baughman Jr.; Leslie Z. Benet

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 0378-4347)

Published

1982

Language

EN

Field

Chemistry (Physical Sciences)