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Alloantigen‐specific T‐cell depletion in a major histocompatibility complex fully mismatched murine model provides effective graft‐versus‐host disease prophylaxis in the presence of lymphoid engraftment by Mickey B. C. Koh; H. Grant Prentice; Marguerite Corbo; Melari Morgan; Finbarr E. Cotter; Mark W. Lowdell is a Medicine article available to read on EtoBox.

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**Summary.** Graft‐versus‐host disease (GvHD) is a multistep immune process involving lymphocyte activation, proliferation and target cell killing. We have devised a novel method for the selective depletion of alloreactive cells from haematopoietic stem cell grafts which retains a pool of immunocompetent lymphocytes possessing antiviral activity with the potential to hasten immune reconstitution. The method is based upon the expression of the activation antigen CD69 on responding donor lymphocytes in a cytokine‐modified mixed lymphocyte culture (mMLC) and depletion of these cells by paramagnetic bead sorting. We have previously demonstrated the __in vitro__ efficacy of this system for the removal of alloreactive cells in both human leucocyte antigen‐mismatched and ‐matched settings. Here, we describe a non‐obese diabetic/severe combined immunodeficient murine model of aggressive GvHD in which we have tested its __in vivo__ efficacy. Murine recipients of infusions of non‐manipulated major histocompatibility complex class I and class II mismatched donor T cells suffered rapid onset of acute, and generally fatal, GvHD. This model is akin to aggressive clinical transfusion‐related GvHD

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Author

Mickey B. C. Koh; H. Grant Prentice; Marguerite Corbo; Melari Morgan; Finbarr E. Cotter; Mark W. Lowdell

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0007-1048)

Published

2002

Language

EN

Field

Medicine (Health Sciences)