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Proteomic and transcriptomic analyses of differential stress/inflammatory responses in mandibular lymph nodes and oropharyngeal tonsils of European wild boars naturally infected with Mycobacterium bovis by Victoria Naranjo; Margarita Villar; Ma Paz Martín-Hernando; Dolors Vidal; Ursula Höfle; Christian Gortazar; Katherine M. Kocan; Jesús Vázquez; José de la Fuente is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Proteomic and transcriptomic analyses of differential stress/inflammatory responses in mandibular lymph nodes and oropharyngeal tonsils of European wild boars naturally infected with Mycobacterium bovis about?

## Abstract Differential stress/inflammatory responses were characterized at the mRNA and protein levels in mandibular lymph nodes (MLN) and oropharyngeal tonsils of European wild boars (__Sus scrofa__), naturally infected with __Mycobacterium bovis__. Suppression‐subtractive hybridization combined with immunohistochemistry and/or quantitative real‐time RT‐PCR were used to identify and characterize abundant stress/inflammatory gene sequences differentially expressed in tuberculous (TB+) wild boars. Genes identified in MLN and tonsils corresponded to serum amyloid A, arginase I, osteopontin, lysozyme, annexin I, and heat shock proteins, respectively. Global protein patterns in MLN and tonsils were compared between TB+ and nontuberculous (TB−) boars by 2‐DE and MALDI‐TOF MS. Five proteins, including stress/inflammatory proteins annexin V, serum albumin, and apolipoprotein A1 were found at lower levels in MLN of TB+ boars. Manganese superoxide dismutase was found up‐regulated in MLN of TB+ boars. Five proteins, including creatine kinase and MHC class II antigens were found up‐regulated in tonsils of TB+ boars. These results demonstrated differential stress/inflammatory responses in wi

Who reads Proteomic and transcriptomic analyses of differential stress/inflammatory responses in mandibular lymph nodes and oropharyngeal tonsils of European wild boars naturally infected with Mycobacterium bovis?

Author

Victoria Naranjo; Margarita Villar; Ma Paz Martín-Hernando; Dolors Vidal; Ursula Höfle; Christian Gortazar; Katherine M. Kocan; Jesús Vázquez; José de la Fuente

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Ltd.; Wiley; Research Square (ISSN 1615-9853)

Published

2007

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)