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Expression of the PEA3 group of ETS-related transcription factors in human breast-cancer cells by Jean-Luc Baert; Didier Monté; Elizabeth A. Musgrove; Olivier Albagli; Robert L. Sutherland; Yvan de Launoit is a Medicine article available to read on EtoBox.
What is Expression of the PEA3 group of ETS-related transcription factors in human breast-cancer cells about?
The PEA3 group of transcription factors belongs to the ets family and is composed of 3 known members, PEA3, ERM and ER81, which are more than 95% identical within the DNAbinding ETS domain and exhibit 50% aa identity overall. Recently, transgenic mice bearing the c-erbB-2/neu oncogene have been shown to over-express PEA3 mRNA in mammary adenocarcinomas, suggesting a role for this gene family in mammary tumorigenesis. In the present work we characterized the mRNA expression levels of PEA3-group genes in a series of human epithelial breast cell lines. Each of the 3 genes was highly expressed in normal human HMEC 1001-7 and HMEC 219-4 cells. In breast-cancer cell lines, the 3 genes were highly expressed in the ER 2 MDA-MB-436, MDA-MB-330, MDA-MB-231 and BT-20 cell lines, but not in the ER 1 MDA-MB-134-VI and ZR-75-1 cells. In an attempt to characterize the PEA3-group proteins in breast-cancer cells, we first produced and characterized specific antibodies against each of these 3 proteins. The anti-ERM and anti-ER81 antibodies recognized specific strong bands at approximately 72 kDa and 62 kDa, corresponding to ERM and ER81, respectively, in MDA-MB-231 and Hs-578T cells expressing signi
Who reads Expression of the PEA3 group of ETS-related transcription factors in human breast-cancer cells?
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- Author
- Jean-Luc Baert; Didier Monté; Elizabeth A. Musgrove; Olivier Albagli; Robert L. Sutherland; Yvan de Launoit
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley; Research Square; Test accounts (ISSN 0020-7136)
- Published
- 1997
- Language
- EN
- Field
- Medicine (Health Sciences)