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Flow Cytometry Detection of Serotonin Content and Release in Resting and Activated Platelets by Giuliana Gobbi; Prisco Mirandola; Pier Luigi Tazzari; Francesca Ricci; Luigi Caimi; Antonio Cacchioli; Stefano Papa; Roberto Conte; Marco Vitale is a Medicine article available to read on EtoBox.
What is Flow Cytometry Detection of Serotonin Content and Release in Resting and Activated Platelets about?
**Summary.** Early detection of platelet activation is important for the diagnosis and follow‐up of several pathological conditions that primarily or secondarily involve platelets in their pathogenesis. The golden standard assay to detect thrombocyte activation is represented by the release of serotonin, classically performed by demanding methodologies, such as high‐performance liquid chromatography, ^14^C‐labelling and enzyme‐linked immunosorbent assay (ELISA). We developed a non‐radioactive method, based on individual cells, for the detection of serotonin content in activated and resting platelets by flow cytometry. The assay was standardized on cells activated by Ca^2+^ ionophore or by sera from patients with heparin‐induced thrombocytopenia (HIT). Cells were identified by CD41a surface staining and their serotonin content measured by specific antiserotonin intracytoplasmic staining, while their activation was independently shown by annexin V binding. Cellular degranulation was detected by flow cytometry in all the cases that were also positive by standard ELISA. Moreover, multiparametric flow cytometry analysis revealed that, although virtually all activated cells bind annexin
Who reads Flow Cytometry Detection of Serotonin Content and Release in Resting and Activated Platelets?
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- Author
- Giuliana Gobbi; Prisco Mirandola; Pier Luigi Tazzari; Francesca Ricci; Luigi Caimi; Antonio Cacchioli; Stefano Papa; Roberto Conte; Marco Vitale
- Publisher
- John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0007-1048)
- Published
- 2003
- Language
- EN
- Field
- Medicine (Health Sciences)